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浅析乙醇提取龙眼核总黄酮及鉴别新研究
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该论文所属栏目导航：医学论文-药学论文
【关键词】 乙醇提取；,,龙眼核；,,总黄酮；,,鉴别
摘要：目的探讨龙眼核中总黄酮的提取及鉴别方法，以充分利用龙眼核植物资源，避免资源的浪费。方法 采用乙醇提取法从龙眼核中提取黄酮类物质，对所提取的黄酮类物质进行验证，并用分光光度法测定其含量。结果测得样品中总黄酮的含量C=1.634mg/ml，回收率为99.65%，其纯度和产率均较高。结论该方法采用全物理过程，无任何污染，是提取龙眼核黄酮类物质的有效途径。
关键词：乙醇提取； 龙眼核； 总黄酮； 鉴别
Abstract:ObjectiveIn order to make use of the resources of longan seed , avoid wasting and approach the extraction of total flavanone of longan seed.MethodsThe flavonids were extracted from longan seed with ethanol .Using spectrophotometry to extract and check the flavanone of longan seed. Results By this method getting the contents of the total flavanone of longan seed is C=1.634mg/ml and the rate of recovery is 99.65%. ConclusionThis text provides the extraction and purifying methods to get the outcome and the purity of the flavanone is all very high .This method is a purely physical process and has not any pollution. It is an ideal way to extract the flavanone of longan seed.
Key words:extracted by ethanol; longan seed; total flavanone; identification
Is often the green fruit tree for the sapindaceae longan，Longan's fruit common name dried longan, also names Long Mu, compared to item, the longan, the Asian litchi chinensis, the longan, the Sichuan billard ball, the pure black horse bead, Yan Luan, the honey spleen,the jiaolei, the wooden bomb, embroiders the wooden group and so on,For our country famous subtropics fruit［1］.The longan originally produces south our country with Vietnam's north South Asia tropics region, had more than 2000 years cultivation history in our country, is the important South Asia tropics often the green longevity fruit tree, is one of four Lingnan good fruits.Li Shi Zhen said in "Native Chinese Plants" that, "Food take litchi chinensis as expensive, but profits take the longan as is good."Longan's nutritional value is very high, is south one of famous good fruits. The longan is the important raw material for medicine.Su Song composes "Chart after Book on Chinese medicine", said longan "Gansu even non-toxic, mainly treats the five internal organs perverse trend, satisfied the mind and pevented the food, long having can strong soul intelligent, enlivens is not old, feel enerthing good".Li Shi Zhen "Native Chinese Plants" also said the longan has "whets the appetite is good for the spleen, makes up the empty longan" the effect.The longan meat may treat the debility of long illness which makes the people feel weakly,Loses sleep , easy to forget,to palpitate with fear, as well as spleen weakness diarrhea, post-natal edema and so on sickness.In addition, the longan shell, the longan nucleus, dragon vertigo and the dragon eyelid, the root may take blends Chinese native medicine.Longan nuclear nature of a medicine astringent, even, is mad micro, taste pale but micro painstakingly, has stops bleeding, stops, regulates the flow of vital energy is wet and so on the effect, uses in treating the wound hemorrhage, the hernia, the mange, the skin disease attributed to dampness and so on［2,3］.The longan nucleus contains the alkaloid and the fatty oil and so on,Except black seed shell,At the end of grinding or fries black at the end of grinding to externally apply,May treat the wound hemorrhage;Or spreads the treatment for burn with the tea oil accent［4］."Compendium Appropriates lost articles" said that, "Every family has the young children, this cannot be unprepared, wounded by cutting, spreads it by this, stops bleeding and lives the muscle, after can produce again , other medicine cannot be copared , not as good as it does."The longan nucleus weight accounts for the longan fruit fresh weight 17%, because has not carried on the comprehensive utilization, abandons every year the longan nuclear weight amounts to several hundred thousand tons, causes the massive longans nucleus to waste in vain［5］.But,At present the total flavanone withdraws the research to the longan nucleus in not to see the report.Therefore, we withdraw the total flavanone for the first time from the longan nucleus, and determines its content, discovered in the longan nucleus includes the massive flavanone ingredient. Now reports as follows.
1 Instrument and reagent
1.1 Instruments KQ5200DB numerical control ultrasonic wave purifier (Kunshan supersonic instrument limited company); UV-755B ultraviolet visible spectrophotometer (Shanghai analysis instrument main plant); ZF-I three uses the ultraviolet analyzer (Shanghai to attend to village photoelectricity instrument plant); SHB-III water-circulation type multifunction vacuum pump (Zhengzhou great wall scientific industry and trade co.ltd ). 800B centrifuge (on Hai'an pavilion scientific instrument factory).
1.2 Reagents 95% Ethyl alcohol; Sodium nitrite AR; Aluminium nitrate AR; Sodium hydroxide AR; Aluminum trichloride AR; Hydrochloric acid AR; Ammonia water AR; Glacial acetic acid AR; Ethyl acetate AR; Magnesium acetate AR; Methyl alcohol AR; Magnesium powder AR; Normal butyl alcohol AR; Standard rutin (Chinese drugs biological preparations examination); Hydrogen peroxide solution AR; Ferrous sulfate AR; Salicylic acid AR. longans nucleus originates from the Guangxi Baise.
2 Methods and result
2.1 Total flavanone ingredients withdraw Take the fresh longan seed, drying, the smashing. The name takes longan seed powder approximately 6g, adds the 80ml 95% ethyl alcohol, the ultrasonic wave withdraws 2.5 h, pulls out filters. The filter residue adds the 40ml 95 % ethyl alcohol again, the ultrasonic wave withdraws 2.5h, pulls out filters, merges two times of filtrate, the reduced pressure recycling ethyl alcohol to filtrates only remains 5~7ml up
摘要：目的探讨龙眼核中总黄酮的提取及鉴别方法，以充分利用龙眼核植物资源，避免资源的浪费。方法 采用乙醇提取法从龙眼核中提取黄酮类物质，对所提取的黄酮类物质进行验证，并用分光光度法测定其含量。结果测得样品中总黄酮的含量C=1.634mg/ml，回收率为99.65%，其纯度和产率均较高。结论该方法采用全物理过程，无任何污染，是提取龙眼核黄酮类物质的有效途径。
关键词：乙醇提取； 龙眼核； 总黄酮； 鉴别
Abstract:ObjectiveIn order to make use of the resources of longan seed , avoid wasting and approach the extraction of total flavanone of longan seed.MethodsThe flavonids were extracted from longan seed with ethanol .Using spectrophotometry to extract and check the flavanone of longan seed. Results By this method getting the contents of the total flavanone of longan seed is C=1.634mg/ml and the rate of recovery is 99.65%. ConclusionThis text provides the extraction and purifying methods to get the outcome and the purity of the flavanone is all very high .This method is a purely physical process and has not any pollution. It is an ideal way to extract the flavanone of longan seed.
Key words:extracted by ethanol; longan seed; total flavanone; identification
Is often the green fruit tree for the sapindaceae longan，Longan's fruit common name dried longan, also names Long Mu, compared to item, the longan, the Asian litchi chinensis, the longan, the Sichuan billard ball, the pure black horse bead, Yan Luan, the honey spleen,the jiaolei, the wooden bomb, embroiders the wooden group and so on,For our country famous subtropics fruit［1］.The longan originally produces south our country with Vietnam's north South Asia tropics region, had more than 2000 years cultivation history in our country, is the important South Asia tropics often the green longevity fruit tree, is one of four Lingnan good fruits.Li Shi Zhen said in "Native Chinese Plants" that, "Food take litchi chinensis as expensive, but profits take the longan as is good."Longan's nutritional value is very high, is south one of famous good fruits. The longan is the important raw material for medicine.Su Song composes "Chart after Book on Chinese medicine", said longan "Gansu even non-toxic, mainly treats the five internal organs perverse trend, satisfied the mind and pevented the food, long having can strong soul intelligent, enlivens is not old, feel enerthing good".Li Shi Zhen "Native Chinese Plants" also said the longan has "whets the appetite is good for the spleen, makes up the empty longan" the effect.The longan meat may treat the debility of long illness which makes the people feel weakly,Loses sleep , easy to forget,to palpitate with fear, as well as spleen weakness diarrhea, post-natal edema and so on sickness.In addition, the longan shell, the longan nucleus, dragon vertigo and the dragon eyelid, the root may take blends Chinese native medicine.Longan nuclear nature of a medicine astringent, even, is mad micro, taste pale but micro painstakingly, has stops bleeding, stops, regulates the flow of vital energy is wet and so on the effect, uses in treating the wound hemorrhage, the hernia, the mange, the skin disease attributed to dampness and so on［2,3］.The longan nucleus contains the alkaloid and the fatty oil and so on,Except black seed shell,At the end of grinding or fries black at the end of grinding to externally apply,May treat the wound hemorrhage;Or spreads the treatment for burn with the tea oil accent［4］."Compendium Appropriates lost articles" said that, "Every family has the young children, this cannot be unprepared, wounded by cutting, spreads it by this, stops bleeding and lives the muscle, after can produce again , other medicine cannot be copared , not as good as it does."The longan nucleus weight accounts for the longan fruit fresh weight 17%, because has not carried on the comprehensive utilization, abandons every year the longan nuclear weight amounts to several hundred thousand tons, causes the massive longans nucleus to waste in vain［5］.But,At present the total flavanone withdraws the research to the longan nucleus in not to see the report.Therefore, we withdraw the total flavanone for the first time from the longan nucleus, and determines its content, discovered in the longan nucleus includes the massive flavanone ingredient. Now reports as follows.
1 Instrument and reagent
1.1 Instruments KQ5200DB numerical control ultrasonic wave purifier (Kunshan supersonic instrument limited company); UV-755B ultraviolet visible spectrophotometer (Shanghai analysis instrument main plant); ZF-I three uses the ultraviolet analyzer (Shanghai to attend to village photoelectricity instrument plant); SHB-III water-circulation type multifunction vacuum pump (Zhengzhou great wall scientific industry and trade co.ltd ). 800B centrifuge (on Hai'an pavilion scientific instrument factory).
1.2 Reagents 95% Ethyl alcohol; Sodium nitrite AR; Aluminium nitrate AR; Sodium hydroxide AR; Aluminum trichloride AR; Hydrochloric acid AR; Ammonia water AR; Glacial acetic acid AR; Ethyl acetate AR; Magnesium acetate AR; Methyl alcohol AR; Magnesium powder AR; Normal butyl alcohol AR; Standard rutin (Chinese drugs biological preparations examination); Hydrogen peroxide solution AR; Ferrous sulfate AR; Salicylic acid AR. longans nucleus originates from the Guangxi Baise.
2 Methods and result
2.1 Total flavanone ingredients withdraw Take the fresh longan seed, drying, the smashing. The name takes longan seed powder approximately 6g, adds the 80ml 95% ethyl alcohol, the ultrasonic wave withdraws 2.5 h, pulls out filters. The filter residue adds the 40ml 95 % ethyl alcohol again, the ultrasonic wave withdraws 2.5h, pulls out filters, merges two times of filtrate, the reduced pressure recycling ethyl alcohol to filtrates only remains 5~7ml up
医学论文-药学论文
